Aktivitas Jus Buah Delima (Punica granatum L.) terhadap Peroksidasi Lipid Darah Tikus yang Diinduksi Parasetamol
DOI:
https://doi.org/10.30736/jev.v2i2.71Abstract
Buah delima atau pomegranat (Punica granatum L.) telah dilaporkan memiliki aktivitas antioksidan lebih tinggi daripada tanaman obat lainnya. Aktivitas antioksidan berpotensi menurunkan kadar lipid peroksida (Tiobarbituric Acid Reacting Substances/TBARS). Tujuan penelitian ini adalah untuk menguji aktivitas jus buah delima terhadap peroksidasi lipid darah tikus yang diinduksi parasetamol. Empat belas ekor tikus dibagi dalam empat kelompok. Kelompok normal (N) dicekok akuades dan tiga kelompok lainnya (kelompok kontrol positif (PC), kelompok kontrol negatif (KN), dan kelompok jus buah delima (JUS) diinduksi parasetamol (500 mg/kgBB) selama 34 hari. Setelah 17 hari, kelompok yang diinduksi parasetamol, yaitu kelompok kontrol positif (PC) diinduksi Cursil 70  dengan dosis (13,3 mg/kgBB), kelompok jus buah delima  diinduksi jus buah delima (JUS) dengan dosis (500 mg/kgBB), tetapi kelompok kontrol negatif (KN) tetap diinduksi parasetamol (500 mg/kgBB) sampai dengan hari ke–34. Lipid peroksida darah direaksikan dengan asam tiobarbiturat (TBA)1% dan dianalisis dengan spektrofotometer pada λ 352 nm. Kontrol negatif (KN) yang diinduksi oleh parasetamol selama 34 hari, meningkatkan TBARS sebesar 120,66%. Kadar lipid peroksida (TBARS) kelompok PC dan kelompok JUS dengan dosis (500 mg/kgBB) menurun secara berturut-turut sebesar 8,14% dan 54,79%.Downloads
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