Deteksi Toxoplasma gondii dari sampel urin dengan Realtime Polymerase Chain Reaction

Fitriana Fitriana

doi:10.30736/5ijev.v5iss1.250

Deteksi Toxoplasma gondii dari sampel urin dengan Realtime Polymerase Chain Reaction

Authors

Fitriana Fitriana

DOI:

https://doi.org/10.30736/5ijev.v5iss1.250

Abstract

Toxoplasma gondii dapat menyebabkan penyakit toksoplasmosis pada manusia. Seropositif yang tidak diobati dapat berkembang menjadi Toksoplasma ensefalitis (sekitar 25%), kerusakan permanen dan kematian. Pengambilan spesimen secara invasif sebagai salah satu kegagalan pengobatan. Hasil uji serologi tidak mudah di interpretasikan pada pasien imunodefisiensi. janin dan bayi. Realtime PCR sebagai metode pilihan untuk menegakkan diagnosis secara tepat dan cepat. Penelitian ini bertujuan mendeteksi T. gondii dengan metode Realtime Polymerase Chain Reaction (Realtime PCR) dari sampel urin. Desain penelitian adalah potong lintang, menggunakan 30 sampel urin penderita HIV/AIDS. DNA di ekstraksi dengan Q1Aamp DNA mini Kit. Realtime PCR Toksoplasma menggunakan enzim RT-PCR Taqman Kit (Bio-Rad). Hasil penelitian PCR dari sampel urin didapatkan 7 sampel (23,3%) terdeteksi T.gondii. Kesimpulannya bahwa metode PCR dapat digunakan untuk mendeteksi T.gondii dari sampel urin pada pasien HIV/ AIDS.

Downloads

Download data is not yet available.

Downloads

PlumX Metrics

Published

2021-03-10

How to Cite

Fitriana, F. (2021). Deteksi Toxoplasma gondii dari sampel urin dengan Realtime Polymerase Chain Reaction. Jurnal EnviScience (Environment Science), 5(1), 33–40. https://doi.org/10.30736/5ijev.v5iss1.250

Download Citation

Issue

Vol. 5 No. 1 (2021)

Section

Artikel

License

Please find the rights and licenses in Jurnal EnviScience (Environment Science). By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.

1. License

The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed onÂ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

2. Author(s)' Warranties

The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User/Public Rights

Jurnal EnviScience's spirit is to disseminate articles published are as free as possible. Under theÂ Creative Commons license, Jurnal EnviScience permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors andÂ Jurnal EnviScience on distributing works in the journal and other media of publications. Unless otherwise stated, the authors are public entities as soon as their articles got published.

4. Rights of Authors

Authors retain all their rights to the published works, such as (but not limited to) the following rights;

Copyright and other proprietary rights relating to the article, such as patent rights,
The right to use the substance of the article in own future works, including lectures and books,
The right to reproduce the article for its own purposes,
The right to self-archive the article (please read out deposit policy),
The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal (Jurnal EnviScience).

5. Co-Authorship

If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. Jurnal EnviScienceÂ will not be held liable for anything that may arise due to the author(s) internal dispute. Jurnal EnviScience will only communicate with the corresponding author.

6. Royalties

Being an open accessed journal and disseminating articles for free under the Creative Commons license term mentioned, author(s) aware that Jurnal EnviScience entitles the author(s) to no royalties or other fees.

7. Miscellaneous

Jurnal EnviScienceÂ will publish the article (or have it published) in the journal if the articleâ€™s editorial process is successfully completed. Jurnal EnviScience's editors may modify the article to a style of punctuation, spelling, capitalization, referencing and usage that deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers as mentioned in point 3.