Detection of Mice DNA in Meatballs Using Real Time – PCR

Triayu Septiani, Pendrianto Pendrianto


Meat is one of perishable food and has the high of water content and protein content. Meatballs are processed food who are using meat as main ingredient and other ingredients, such tapioca flour, salt, pepper, and the other ingredients as food additives. Meatballs is the mostpopular because has tasty flavor, high protein content, especiallyessential amino acids.In Indonesia, until now there were still cases of counterfeiting of processed meat products. One of method is mixing the beef meat and other animalsmeat in the making process to reduce production costs, so that similar products can be obtained at a much lower cost. One common case is the mixing of beef meat with rat meat. This study aims to identify meatballs contaminated by rat meat. Meatball samples used do not have Halal certificate from LPPOM MUI. From the results of the study, obtained the green amplification curve with the highest peak showed positive control of rat DNA, followed by three other green curves from the sample that showed the occurrence of amplification, thus indicating samples positive containing rat DNA. In the positive sample, the rat DNA concentration in the sample is quite low which can be seen from the intensity of the peak produced on the amplification curve when compared with positive control.


Keywords :DNA, Meat,Meatballs, Rat, RT – PCR


Ayu, B.P., Purbowatiningrum, R.S., Agustina, L.N.A. 2011. Purifikasi DNA Kromosom Geobacillus sp dYTae-14 Menggunakan Kolom Silika dengan Denaturan Urea. Jurnal Sains dan Matematika Vol. 19 (4): 101 – 106 (2011).

Aziz, A.Y. and Vui, C.N. 2012. The Role of Halal Awareness and Halal Certification in Influencing Non-Muslim’s Purchase Intention. University Putra Malaysia. Third International Conference on Business and Economic Research (3rd ICBER 2012) Proceeding, ISBN: 978-967-5705-05-2.

Bohari, A. M., Hin, C. W. and Fuad, N. 2013. The Competitiveness of Halal Food Industry in Malaysia : A SWOT ICT Analysis. Geografia Online Malaysia Journal of Society and Space 9 issue 1 (1 - 9) 1, ISSN 2180-2491.

Heinz, G., Hautzinger, P. 2007. Meat Processing Technology For Small To Medium Scale Producers. FAO Regional Office For Asia and The Pacific, RAP Publication 2007 / 20.

Farag, M.R., Alagawany, M., El-Hack, M. E. A., Tiwari, R., Dhama, K. 2015. Identification of Different Animal Species in Meat and Meat Products: Trends and Advances. Jour. of Adv. Anim. Vet. Sci. 3(6): 334 – 346.

Girindra, Aisjah. (2006). Menjamin Kehalalan dengan Label Halal. Persfektif Food Review Indonesia Vol.1 No 9. hal.12-13. Bogor.

Ilhak, O.I., Arslan, A. 2007. Identification of Meat Species by Polymerase Chain Reaction (PCR) Technique. Turk. J. Vet. Anim. Sci: 31(3): 159 – 163.

Janosi, A. 2006. Species Specific Detection of Meat by Polymerase Chain Reaction Techniques. Corvinus University of Budapest, Budapest, Rumania.

Joshi, M., Deshpande, J.D. 2010. Polymerase Chain Reaction: Methods, Principles and Application. International Journal of Biomedical Research 1 (5) 81 – 97.

Jonker, K.M., Tilburg, J.J.H.C., Hagele, G.H., Boer, E.D. 2008. Species Identification in Meat Products Using Real – Time PCR. Food Aditives and Contaminants: Part A, 25:5, 527 – 533.

Margawati, E.T., Ridwan, M. 2010 Pengujian Pencemaran Daging Babi Pada Beberapa Produk Bakso Dengan Teknologi PCR: Pencarian Sistem Pengujian Efektif. Jurnal Ilmu – Ilmu Hayati : Berita Biologi, Vol 10 (1): 93 – 98 (2010).

Mursyidi, A. 2012. The Role Of Chemical Analysis in the Halal Authentication of Food and Pharmaceutical Products. J. Food Phar.Sci. 1 (2013) 1 – 4.

Murugaiah, C., Noor, Z.M., Mastakim, M., Bilung, L.M., Selamat, J., Radu, S. 2009. Meat Species Identification and Halal Authentication Analysis Using mitochondrial DNA. Elsevier Journal of Meat Science 83 (2009) 57 – 61.

Pascoal, A., Prado, M., Castro, J., Cepeda, A. 2004. Survey of Authenticity of Meat Species in Food Products Subjected to Different Technological Processes, of PCR - RFLP Analysis. Eur. Food Res. Technol. 218: 306 -312.

Sahilah, A.M., Norhayati, Y., Norrakiah, A. S., Aminah, A. and Wan Aida, W. M. 2011. Halal Authentication of Raw Meats Using PCR Amplification of Mitochondrial DNA. International Food Research Journal 18(4): 1489-1491 (2011)

Salehudin, I. 2010. Halal Literacy: A Concept Exploration and Measurement Validation. Dept. of Management, Faculty of Economics, University of Indonesia. Asean Marketing Journal, June 2010 – Vol. II – No. 1.

Srihanto, Setiaji, E.A., Rumpaka, G., Firwantoni. 2011. Identifikasi Daging Tikus Pada Produk Asal Hewan Dengan Menggunakan Teknik Polimerase Chain Reaction (PCR). Prosiding Seminar Nasional Sains dan Inovasi Teknologi Pertanian.

Unajak, S., Meesawat, P., Anyamaneeratch, K. Anuwareepong, D., Srikulnath, K., Choowongkomon, K. 2011. Identification of Species (Meat and Blood Samples) Using Nested-PCR Analysis of Mitochondrial DNA. African Journal of Biotechnology Vol. 10(29), pp. 5670-5676, 22 June, 2011.

Wardani, A.K. dan Sari, E. P. K. 2015. Deteksi Molekuler Cemaran Daging Babi Pada Bakso Sapi Di Pasar Tradisional Kota Malang Menggunakan PCR. Jurnal Pangan dan Agroindustri Vol.3 No 4 p. 1294 – 1301, September 2015.

Widyasari, Y. I., Sudjadi., Rohman, A. 2015. Detection of Rat Meat Adulteration in Meat Ball Formulations Employing Real Time PCR. Asian Journal of Animal Sciences 9 (6): 460 – 465, 2015.

Zidek, R., Bajzik, P., Marsalkova, L., Golian, J. Detection of Counterfeit Beef Using Real – Time PCR. 2011. Dept. of Hygiene and Food Safety, Slovak University of Agriculture in Nitra.



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