Qualitative and Quantitative Analysis of Hydroquinone in Face Cream with TLC and HPLC Methods


  • Rahma Dona Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau) Jl.Kamboja Simpang Baru Panam- Pekanbaru- Riau
  • Selvia Selvia




Hydroquinone, Qualitative, Quantitative, TLC, HPLC


Hydroquinone is a chemical compound widely used in cosmetics that acts as a removal of black spots/spots on the face. The use of hydroquinone in cosmetics, according to BPOM regulations, is only allowed with a maximum level of 2%. Excessive use of hydroquinone can cause pigment abnormalities in the face. The materials used in this study were hydroquinone BPFI, 96% ethanol, absolute ethanol, sodium sulfate, methanol (HPLC grade), aquadest, ethyl acetate, and face cream samples (X and Y). Qualitative and quantitative analysis of hydroquinone content in both face cream samples was carried out by TLC and HPLC methods. The results of the qualitative analysis by TLC on both face cream samples (X and Y creams) showed that both samples had the same Rf value 1as the standard hydroquinone Rf while the HPLC chromatogram results of both samples had the same retention time as the standard hydroquinone. Simultaneously, the results of quantitative analysis on cream X samples contained hydroquinone of 3.06% and Y cream of 4.15%. From these results, both face cream samples positive contained hydroquinone with levels> 2%.


Download data is not yet available.

Author Biography

Rahma Dona, Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau) Jl.Kamboja Simpang Baru Panam- Pekanbaru- Riau

Program Studi Sarjana farmasi STIFAR Riau


Zukepli M, Wihdah N, Omar W, Atikah WS, Zakaria, Raihan S. Assessment on Hyroquinone in Selected Cosmetic Cream and Toner via High Performance Liquid Chromatography and Ultra-Violet Visible Detector Spectrometry. Malaysian Journal of Analytical Sciences 2015; 19: 824–830.

BPOM RI. Kosmetik Mengandung Bahan Berbahaya dan Zat Warna Yang Dilarang: Keputusan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia No. HK.00.01.432.6081. 2007.

Sigma-Aldrich. Hydroquinone ReagentPlus®, ≥99%. 2018.https://www.sigmaaldrich.com/catalog/product/aldrich/240125?lang=en&region=ID .

Commission Regulation (EU). Regulations No. 344/2013 of 4 April 2013 Amending Annexes II, III, V and VI to Regulation (EC) No 1223/2009 of the European Parliament and the Council on Cosmetic Products. OJEU, 2013, L114/1. 2013. 2013.http://www.eurlex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2013:114:0001:0059:EN:PDF.

Anief M. Farmasetika. UGM Press: Yogyakarta, 2012.

BPOM RI. Public Warning / Peringatan Nomor KH. 00.01.433.6147 Tentang Kosmetik Mengandung Bahan Berbahaya Dan Zat Warna Yang Dilarang. 2007.

Gimeno P, Maggio AF, Bancilhon M, Lassu N, Gornes H, Brenier C et al. HPLC-UV method for the identification and screening of hydroquinone, ethers of hydroquinone and corticosteroids possibly used as skin-whitening agents in illicit cosmetic products. Journal of Chromatographic Science 2016; 54: 343–352.

Thongchai W, Liawruangrath B, Liawruangrath S. High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts. Journal of Cosmetic Science 2007; 58: 35–44.

Westerhof W, Kooyers TJ. Hydroquinone and its analogues in dermatology - a potential health risk. Journal of Cosmetic Dermatology 2005; 4: 55–59.

Rohman A. Kromatografi untuk analisis obat. 1st ed. Graha Ilmu: Yogyakarta, 2009.

Lin WC, Lin ST, Shu SL. Comparison of analyses of surfactants in cosmetics using high-performance liquid chromatography and high-performance capillary electrophoresis. Journal of Surfactants and Detergents 2000; 3: 67–72.

Gianti. Analisis Kandungan Merkuri Dan Hidrokuinon Dalam Kosmetik Krim Racikan Dokter. FKM UIN Hidayatullah Jakarta. 2013.


PlumX Metrics



How to Cite

Dona, R., & Selvia, S. (2020). Qualitative and Quantitative Analysis of Hydroquinone in Face Cream with TLC and HPLC Methods. Jurnal Midpro, 12(2), 157–162. https://doi.org/10.30736/md.v12i2.175